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| Registros recuperados : 15 | |
| 4. |  | ALMEIDA, A. M. R.; MITUTI, T.; BELINTANI, P.; GASPAR, J. O.; KITAJIMA, E. W. Identificação e caracterização de um carlavirus em Arachys repens Hando. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 310-311. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 5. | | ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; BELINTANI, P.; KITAJIMA, E. W. Caracterização bio-molecular do Tobacco streak virus causador da queima do broto da soja no Brasil. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 308-309. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 6. | | BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. R.; KITAJIMA, E. W. Partial sequencing of Cowpea mild mottle virus - CPMMV, isolate of Barreiras - Bahia. Virus Reviews & Research, Rio de Janeiro, v. 10, p. 31, Nov. 2005. Supplement. Edição dos Resumos da XVI National Meeting of Virology, nov. 2005.| Biblioteca(s): Embrapa Soja. |
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| 7. | | GASPAR, J. O.; BELINTANI, P.; ALMEIDA, A. M. R.; KITAJIMA, E. W. A primer pair allows amplification of part of the 3'-terminal of carlaviruses genome. Virus Reviews & Research, Rio de Janeiro, v. 10, p. 140, Nov. 2005. Supplement, ref. P-191. Edição dos Resumos da XVI National Meeting of Virology, nov. 2005.| Biblioteca(s): Embrapa Soja. |
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| 8. | | BELINTANI, P. GASPAR, J. O.; MITUTI, T.; ALMEIDA, A. M.; KITAJIMA, E. W. Sequenciamento parcial e identificação de um carlavirus obtido de Arachys repens. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S 191, ago. 2005. Suplemento. ref. 815. Edição dos Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005. Nome correto do quarto autor: ALMEIDA, A. M. R.| Biblioteca(s): Embrapa Soja. |
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| 9. | | MITUTI, T.; CORTEZI, D.; NUNES, C. L.; KITAJIMA, E. W.; BELINTANI, P.; GASPARJ. O.; ALMEIDA, A. M. R. Identificação de um isolado de carlavirus em Arachis pintoi Krap & Greg. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2005, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2005. p. 140-144. (Embrapa Soja. Documentos, 268).| Biblioteca(s): Embrapa Soja. |
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| 10. | | MITUTI, T.; CORTEZI, D. G.; NUNES, C. L.; KITAJIMA, E. W.; BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. Identificação de um isolado de carlavirus em Arachis repens Handro. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S 187, ago. 2005. Suplemento. ref. 788. Edição dos Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005. Nome correto do sétimo autor: ALMEIDA, A. M. R.| Biblioteca(s): Embrapa Soja. |
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| 11. | | ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; OLIVEIRA, T. G.; BELINTANI, P.; KITAJIMA, E. W.; NORA, P. S.; NOVAES, T. G. Biological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 229-230. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 12. | | ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S. Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. Fitopatologia Brasileira, Brasília, v. 30, n. 4, p. 366-373, July/Aug. 2005.| Biblioteca(s): Embrapa Soja. |
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| 13. | | ALMEIDA, A. M. R.; PIUGA, F. F.; KITAJIMA, E. W.; GASPAR, J. O.; VALENTIN, N.; BENATO, L. C.; MARIN, S. R. R.; BINNECK, E.; OLIVEIRA, T. G.; BELINTANI, P.; GUERZONI, R. A.; NUNES JR. J.; HOFFMANN, L.; NORA, P. S.; NEPOMUCENO, A. L.; MEYER, M. C.; ALMEIDA, L. A. Necrose da haste da soja. Londrina, PR: Embrapa Soja, 2003. 44 p. (Embrapa Soja. Documentos, 221).| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Cerrados; Embrapa Unidades Centrais. |
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| 14. | | ALMEIDA, A. M. R.; PIUGA, F. F.; KITAJIMA, E. W.; GASPAR, J. O.; VALENTIN, N.; BENATO, L. C.; MARIN, S. R. R.; BINNECK, E.; OLIVEIRA, T. G. de; BELINTANI, P.; GUERZONI, R. A.; NUNES JUNIOR, J.; HOFFMANN, L.; NORA, P. S.; NEPOMUCENO, A. L.; MEYER, M. C. Necrose da haste da soja. Londrina: Embrapa Soja, 2003. 44 p. (Embrapa Soja. Documentos, 221).| Biblioteca(s): Embrapa Trigo. |
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| 15. | | ALMEIDA, A. M. R.; PIUGA, F. F.; KITAJIMA, E. W.; GASPAR, J. O.; VALENTIN, N.; BENATO, L. C.; MARIN, S. R. R.; BINNECK, E.; OLIVEIRA, T. G. de; BELINTANI, P.; GUERZONI, R. A.; NUNES JUNIOR, J.; HOFFMANN, L.; NORA, P. S.; NEPOMUCENO, A. L.; MEYER, M. C.; ALMEIDA, L. A. Necrose da haste da soja. Londrina: Embrapa Soja, 2003. 44 p. (Embrapa Soja. Documentos, 221).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 15 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/12/2005 |
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Data da última atualização: |
02/12/2005 |
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Autoria: |
BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. R.; KITAJIMA, E. W. |
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Título: |
Partial sequencing of Cowpea mild mottle virus - CPMMV, isolate of Barreiras - Bahia. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Virus Reviews & Research, Rio de Janeiro, v. 10, p. 31, Nov. 2005. Supplement. |
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Idioma: |
Inglês |
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Notas: |
Edição dos Resumos da XVI National Meeting of Virology, nov. 2005. |
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Conteúdo: |
In recent years, a viral diseases on soybeans called "necrose da haste" has become a serious problem in West Central Brazil, causing principal dwarfing and stem necrosis in soybean. Based on transmission, purification and serology, electron microscopy and molecular studies the causal agent was determined to be a whitfly-borne carlavirus which is possibly related to Cowpea mild mottle virus (CpMMV) (Almeida et al. Fitop. Bras. 30:191-194. 2005). CpMMV has already been identified in Brazil causing mosaic in bean cv. 'Jalo' (Costa et al. Fitop. Bras. 8:325-337. 1983), however, the symptoms observed recently are much more serious causing severe stem necrosis and death of the plants, devastating soybean fields in different regions (States of Goiás, Mato Grosso, Bahia and Maranhão and, more recently, in State of Paraná - South Brazil). Infected soybean plants material were collected from the field, at Barreiras, in the State of Bahia and multiplied for the molecular analyses. Aiming to confirm the identity the isolated virus, which has a 8,3kb ssRNA genome, the 3'-terminal region of the virus genome isolated was cloned and sequencend. The sequence encompasses incomplete six open reading frames (ORFs) and a polyadenilated tail. Analysis of ORF1 indicated that this portion of viral genome shares significant similary to the C-terminus of the carlavirus replicase (68-73%). The three partially overlapping ORFs 2, 3 and e refered to as the "triple gene block" (TGB), juxtaposed between the replicase gene and the viral coat protein (CP) gene indicated greater identity, around 50%, to CPMMV isolates already sequenced (CPMMV H-AF02628 and CPMMV M-AF024629) and also significant identity, although, lower, with other carlavirus. Similary, ORFs 5 and 6, when compared with CPMMV isolates (AF024628), AF024629) showed high identity to the coat protein gene (89-91,3%) and to a carlavirus protein with potential nucleic acid binding property (50-52,%). Phylogenetic analyses and the presence of characteristic domains in putative protein genes of virus isolate at Barreiras - Bahia had demonstrated the highest homology with members of Carlavirus genus, and more specifically with CPMMV. Molecular diagnosis test are now available for CPMMV using specific primers designs to RT-PCR amplifying a 1300bp fragment including a CP and 11K ORFs. MenosIn recent years, a viral diseases on soybeans called "necrose da haste" has become a serious problem in West Central Brazil, causing principal dwarfing and stem necrosis in soybean. Based on transmission, purification and serology, electron microscopy and molecular studies the causal agent was determined to be a whitfly-borne carlavirus which is possibly related to Cowpea mild mottle virus (CpMMV) (Almeida et al. Fitop. Bras. 30:191-194. 2005). CpMMV has already been identified in Brazil causing mosaic in bean cv. 'Jalo' (Costa et al. Fitop. Bras. 8:325-337. 1983), however, the symptoms observed recently are much more serious causing severe stem necrosis and death of the plants, devastating soybean fields in different regions (States of Goiás, Mato Grosso, Bahia and Maranhão and, more recently, in State of Paraná - South Brazil). Infected soybean plants material were collected from the field, at Barreiras, in the State of Bahia and multiplied for the molecular analyses. Aiming to confirm the identity the isolated virus, which has a 8,3kb ssRNA genome, the 3'-terminal region of the virus genome isolated was cloned and sequencend. The sequence encompasses incomplete six open reading frames (ORFs) and a polyadenilated tail. Analysis of ORF1 indicated that this portion of viral genome shares significant similary to the C-terminus of the carlavirus replicase (68-73%). The three partially overlapping ORFs 2, 3 and e refered to as the "triple gene block" (TGB), juxtaposed between t... Mostrar Tudo |
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LEADER 02918naa a2200169 a 4500
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100 1 $aBELINTANI, P.
245 $aPartial sequencing of Cowpea mild mottle virus - CPMMV, isolate of Barreiras - Bahia.
260 $c2005
500 $aEdição dos Resumos da XVI National Meeting of Virology, nov. 2005.
520 $aIn recent years, a viral diseases on soybeans called "necrose da haste" has become a serious problem in West Central Brazil, causing principal dwarfing and stem necrosis in soybean. Based on transmission, purification and serology, electron microscopy and molecular studies the causal agent was determined to be a whitfly-borne carlavirus which is possibly related to Cowpea mild mottle virus (CpMMV) (Almeida et al. Fitop. Bras. 30:191-194. 2005). CpMMV has already been identified in Brazil causing mosaic in bean cv. 'Jalo' (Costa et al. Fitop. Bras. 8:325-337. 1983), however, the symptoms observed recently are much more serious causing severe stem necrosis and death of the plants, devastating soybean fields in different regions (States of Goiás, Mato Grosso, Bahia and Maranhão and, more recently, in State of Paraná - South Brazil). Infected soybean plants material were collected from the field, at Barreiras, in the State of Bahia and multiplied for the molecular analyses. Aiming to confirm the identity the isolated virus, which has a 8,3kb ssRNA genome, the 3'-terminal region of the virus genome isolated was cloned and sequencend. The sequence encompasses incomplete six open reading frames (ORFs) and a polyadenilated tail. Analysis of ORF1 indicated that this portion of viral genome shares significant similary to the C-terminus of the carlavirus replicase (68-73%). The three partially overlapping ORFs 2, 3 and e refered to as the "triple gene block" (TGB), juxtaposed between the replicase gene and the viral coat protein (CP) gene indicated greater identity, around 50%, to CPMMV isolates already sequenced (CPMMV H-AF02628 and CPMMV M-AF024629) and also significant identity, although, lower, with other carlavirus. Similary, ORFs 5 and 6, when compared with CPMMV isolates (AF024628), AF024629) showed high identity to the coat protein gene (89-91,3%) and to a carlavirus protein with potential nucleic acid binding property (50-52,%). Phylogenetic analyses and the presence of characteristic domains in putative protein genes of virus isolate at Barreiras - Bahia had demonstrated the highest homology with members of Carlavirus genus, and more specifically with CPMMV. Molecular diagnosis test are now available for CPMMV using specific primers designs to RT-PCR amplifying a 1300bp fragment including a CP and 11K ORFs.
700 1 $aGASPAR, J. O.
700 1 $aALMEIDA, A. M. R.
700 1 $aKITAJIMA, E. W.
773 $tVirus Reviews & Research, Rio de Janeiro$gv. 10, p. 31, Nov. 2005. Supplement.
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